Phic31 integrase vector system

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August undefined, 2024

WebThe PhiC31 Integrase System is an non-viral, integrase-based gene expression system for low- or single-copy integrations up to large insert sizes. The PhiC31 Systems comes in a … WebMay 12, 2014 · Vector constructs used in this study. (A and B) The genetic structure of the barnase expression vector (“pro-vector”) (A) and the phiC31-integrase-expression vector (B). The provector harbors attP and attB sequences that serve as targets for the site-specific recombinase phiC31. Note that only the T-DNA part of the vectors is illustrated ...

Improved site-specific recombinase-based method to produce

WebA polymerase chain reaction assay is also presented to verify correct chromosomal placement of the introduced plasmid. This integration system based on phiC31 integrase supplies a simple method to obtain repeated integration at the same chromosomal site in mammalian cells. Publication types Research Support, N.I.H., Extramural MeSH terms Webprotein (EGFP) positive and phiC31 integrase could recombine the reporter gene in a site specific manner. These results demonstrate that attP site can be used as a proper location to conduct site directed transgenesis in both mammalian cells and embryos in phiC31 integrase system when even combinaed to SCNT and intracytoplasmic sperm hazmat security awareness training

Construction of a Mammalian IRES-based Expression Vector to

WebJan 1, 2007 · The phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid … WebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An … WebSep 14, 2012 · Abbreviations: phiC31, phage phiC31 recombinase coding sequence [41]; Pubi, maize ubiquitin 1 promoter; Pspm, promoter of the maize suppressor-mutator transposable element spm; intron, sequence derived from an intron of the Petunia hybrida Psk7 gene (GenBank accession number AJ224165); NLS, SV40 T antigen nuclear … hazmat security clearance hawaii

phiC31-integrase-mediated, site-specific integration of ... - Springer

Nucleofection of phiC31 Integrase Protein Mediates

WebphiC31 Integrase Vector System pFC-PGK-MCS-EF1-GFP-Puro Dual Promoter phiC31 Donor Vector (Cat# FC551A-1). The cells were split 1:10 after 24 hours post-transfection. … WebOver the past decade, the integrase enzyme from phage phiC31 has proven to be a useful genome engineering tool in a wide variety of species, including mammalian cells. hazmat security awareness training videoWebJun 1, 2024 · In this study, we have compared the mAb0014 expression level of single ORF/IRES vector and dual ORF vector in the presence and absence of phiC31 integrase … golang common.isempty

"WebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … " - Phic31 integrase vector system

Phic31 integrase vector system

PhiC31 integrase can improve the efficiency of ... - ScienceDirect

WebJun 1, 2024 · As one of the first groups using phiC31 in mab expression [25], we have prepared two different designs for mammalian expression vectors, which include: single … WebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into …

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WebOur results demonstrated that dual ORF vector in the presence of phiC31 integrase expression vectors (+FC31 2P) generated more recombinant antibody in comparison to … WebDec 9, 2024 · This vector system consists of several modules including a Tet-inducible promoter directing the expression of a transgene and two Tet repressor expression units placed in tandem on a single vector. The cell clones, generated using a one-step phiC31 integrase-mediated chromosomal integration of the multi-cDNA expression construct, …

WebJun 12, 2024 · This study demonstrates that phiC31 integrase protein, whether in the 605- or 613-amino-acid form, is competent to mediate genomic integration when co-nucleofected with a donor plasmid. Both forms of the protein mediated sequence-specific integration, with a tendency for the 613-amino-acid form to be more efficient. WebIn this study, phiC31 integrase technology in combination with DHFR amplification system was used to have an expression vector for future expression of blinatumomab in CHO cells. The gene of interest (BsAb gene) could be joined to DHFR selection marker with the insertion of an internal ribosome entry site (IRES).

WebIn addition, combining PhiC31 integrase with R4 integrase allows for isogenic expression from a defined genomic locus, providing the ideal solution for comparative analysis of gene families, isoforms or orthologs. ... Jump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material ... WebINTRODUCTION. The Streptomyces phage phiC31 uses a large serine recombinase, phiC31 integrase (Int), to merge its genome with that of its bacterial host [1, 2].Its two substrates …

WebOct 25, 2007 · Although this system has proven useful, limited payload capacity and the potential for gene disruption from random integration of DNA could limit its utility in stem cells. ... We typically used 4 μg of the expression vector and 4 μg of the phiC31 integrase expression vector to transfect 2 million cells. Control transfections omitted the ...

WebFeb 20, 2013 · The aim of present study was cloning and expression of phiC31 integrase cDNA in a bacterial expression vector. Thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. Materials and Methods: hazmat secondary containmentWebJun 1, 2024 · Co-transfection of dual ORF vector with PhiC31 integrase expression vector resulted in FC31 2P cell pools. As the negative control, cells transfected just with single-ORF/IRES or dual-ORF vectors, without phiC31 integrase expression vector. A total amount of 3 μg plasmid DNA was used for each well of the 6-well plate. golang comparing slicesWebJul 25, 2024 · The PhiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage PhiC31. The PhiC31 integrase mediates precise, unidirectional recombination between two att sites named attB and attP. This integrase has been shown to function efficiently in many different cell types as well as in mice [ 12, 13 ]. golang complex128 golang compare empty structWebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) A memory switch for … hazmat security programWebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Contact Us - PhiC31 Integrase Expression Plasmid System Biosciences Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … NTA-based Exosome Characterization - PhiC31 Integrase Expression Plasmid … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … CLOuD9 Gene Expression Regulation System; Targeted Protein Degradation; … Lentiviral Packaging Kits - PhiC31 Integrase Expression Plasmid System Biosciences How it works. Ordering RNA Services is easy. Step 1: Contact the services team … Exosome Biomarker Discovery Products - PhiC31 Integrase Expression Plasmid … Get everything you need for studying and using exosomes, from isolation and … hazmat security awareness training.pptWebJun 1, 2024 · Our results demonstrated that dual ORF vector in the presence of phiC31 integrase expression vectors (+FC31 2P) generated more recombinant antibody in comparison to its negative control (-FC31 2P). Moreover, both of +FC31 2P and -FC31 2P cell pools yield higher recombinant protein in comparison to single ORF/IRES vector (FC31 … hazmat service life