Cisplatin ic50 a549

WebNov 19, 2013 · A549 and DU145 cells were exposed to cisplatin at an IC50 dose (12 μM and 20 μM, respectively) and ROS levels were measured at the indicated time points by incubating with H 2 DCFDA or MitoSox fluorescent probes. ROS levels in treated vs. non treated cells at each time point were analyzed independently for each probe by two-way … WebThe IC50 value of cisplatin was also assessed with CCK-8 assays. The western blotting for AKT, p-AKT, S6K and p-S6K were performed in cells with TAZ knockdown or overexpression. When the AKT/mTOR pathway was blocked in A549 and H460 with TAZ overexpression, cisplatin sensitivity was assessed by IC50 value.

Reversal of resistance to cisplatin in NSCLC by miRNA-195-5p

WebDec 29, 2009 · Fig. 3B confirms that, unlike cisplatin, a conventional chemotherapeutic agent, mitaplatin selectively induced cell death in human cancer A549 cells, but not in normal MRC-5 cells under the similar treatment conditions. To address whether the selective killing of cancer cells by mitaplatin might be a consequence of its selective uptake, we ... WebMar 17, 2024 · To establish the cisplatin-resistant cell line, A549-R, A549 cells were treated with a stepwise increasing concentration of cisplatin (Sigma-Aldrich, USA) as previously described. 29, 30 The CAFs and normal fibroblasts (NFs) were isolated from NSCLC tissues and adjacent normal tissues by primary culture, as previously described, respectively. … dailymotion scene https://pillowtopmarketing.com

Metformin impairs cisplatin resistance effects in A549 lung cancer ...

WebNext, the highest cisplatin IC50 was found in CAFs, and A549 cells had the lowest IC50 value . The ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ... WebJan 3, 2003 · The concentration of Gleevec to inhibit 50% cell growth (IC50) was estimated to be around 2–3 μM (Figure 1), whereas the IC50 of cisplatin on A549 cells was … WebIn A549 cells, the IC 50 concentration of cisplatin- resistant cells was determined to be 43.01 µM compared to 6.14 µM in the original parental cell line; a 7-fold increase in the concentration... dailymotion world at war episode 14

Cisplatin induces apoptosis of A549 cells by …

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Cisplatin ic50 a549

Circular RNA hsa_circ_0096157 contributes to cisplatin resistance …

WebMay 11, 2016 · A549 cells. After 156 hrs of monitoring, the diameter of A549 spheroid controls decreased by a total of 141 μM. During that time, spheroids treated with 20 and 40 μM of DXR also decreased, shrinking by −206 μM and −178 μM, respectively, the rate of shrinkage was faster in the treated spheroids compared with the controls. WebFor A549, except for II, the other complexes exhibited moderate cytotoxicity. For non-tumorigenic ligeages, the complexes were less toxic than cisplatin for MCF-10A, with emphasis on V, which was inactive. In addition, all complexes were more selective than cisplatin at A2780-cis and for MCF-7, V exhibited selectivity index > 6.

Cisplatin ic50 a549

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WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for …

WebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 ... USA; CRM‐CCL‐185), A549 cisplatin‐resistant (A549/DDP; National Infrastructure of Cell Line Resource, Beijing, China; 1101HUM‐PUMC000519), and human lung bronchial epithelial 16HBE cells (Shanghai Fuheng Biotechnology Co., Ltd. China; … WebApr 8, 2024 · Lung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated the role of mTOR signaling and other signaling pathways after metformin (MET) treatment in control and cisplatin‑resistant A549 cells, mapping pathways and possible …

WebWith the treatment prolongation of 4 μmol/L Cisplatin in A549 cells, Bcl-2 and caspase-3 were downregulated, while BAD upregulated. Conclusions: Cisplatin treatment induces … WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were …

WebDec 1, 2024 · microRNA IC50 inhibit concentration by 50% Keywords Colorectal neoplasiaa Drug resistance Oxaliplatin ABCB1 GSTP1 miRNA 1. Introduction Colorectal cancer is one of the major fatal malignancies, which is the third and second most prevalent cancer in males and females respectively.

WebLung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated … dailymotion heidi folge 38WebMar 22, 2024 · The drug’s IC 50 values indicated that the cells had acquired a stable CDDP-resistance phenotype, with the IC 50 values of the A549/DDP cells remaining … dailyom 21 day yoga reviewsWebThe IC50 values are slightly higher than those previously reported in the literature for cisplatin, 5.7 ± 0.1 (incubation for 24 h) and 14.4 ± 1.1 (incubation for 48 h) for MCF-7 [64] and PANC-1 ... dailymotion sherwoodWebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi … dailymotion the brady girls get marriedWebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … dainty myoWebThe IC 50 of cisplatin on A549 cells was 4.07±0.32, 2.26±0.43, and 1.52±0.41 µg/mL, for 24, 48, and 72 hours, respectively. Thus, we used 4, 2, and 1 µg/mL of cisplatin to treat A549 cells... dainty dumpling house mirandaWebJan 3, 2003 · We used the IC50 concentrations for both Gleevec and cisplatin to treat A549 cells in culture, and assessed the viability of the cells by MTT assays at the different time points (Figure 4 ). Gleevec (2 μM) and cisplatin (64 μM) both inhibited the growth of the tumor cells individually after treatment of the cells for 48 hour. daintree family dental clinic