WebThis product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and … WebAug 13, 2012 · Genetic transformation is a natural process during which foreign DNA enters a cell and integrates into the genome. ... 150 µl PLi [4.5 ml PEG 50%, 610 µl LiAc 1M, 57 µl Tris 1M (pH 7.5), 11.6 µl EDTA 1M (pH 7.4)], 2.5 µl boiled ss-carrier DNA (1 mg/ml) and 10 µg DNA (PCR product). The plate was incubated in a water bath at 42°C for 30 ...

Salmon Sperm DNA Solution - Thermo Fisher Scientific

WebFeb 29, 2016 · Each reaction was composed of 2.1 OD 600 of cells (3 ml of cells at 0.7–0.8 OD 600), 120 μl of 50% PEG 3500 (wt/vol), 18 μl of 1 M LiAc, 25 μl of boiled SS-carrier DNA, 7 μl of double ... WebYeast transformation by the LiAc/SS carrier DNA/PEG method. Efficient transformation of yeast has been the corner stone of molecular studies in yeast for numerous years. It is … insurrection south africa

Yeast Transformation by the LiAc/SS Carrier DNA/PEG …

WebJan 1, 2014 · Samples should be stored at −20 °C. Carrier DNA should be denatured in a boiling water bath for 5 min and chilled immediately in an ice/water bath before use. Denatured carrier DNA can be boiled three … WebJan 31, 2007 · Denatured carrier DNA can be boiled three or four times without significant loss of activity. Equipment setup Glassware 250 ml baffled Erlenmyer flask, sterile (Fisher Scientific Ltd, cat. no. 10 ... WebBoil a 1.0mL sample of SS-DNA for 5 min. and quickly chill in ice water. It is not necessary or desirable to boil the carrier DNA every time. Keep a small aliquot in your own freezer box and boil after 3-4 freeze-thaws. insurrection speech