WebBioFact 2X Multi-Star PCR Series; BioFact All-in PCR Cloning Kit; BioFact DNA Ladders; BioFact F-Star Taq DNA Polymerase; BioFact Gel & PCR Purification System; BioFact … WebThe cDNA was then used as a template in qRT PCR to study the transcript accumulation of selected genes (Table S1) using a real-time PCR master mix including SYBR green (BioFact, Korea) along with ...
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WebDec 3, 2024 · Real-time PCR was performed using the SYBR Green Master Kit (BioFACT, Korea), which emits fluorescence to cDNA that can then be detected. Each sample was assayed in triplicate. The Bio-Rad CFX connects Real-time system was used for analysis with the following program: initial denaturation at 95°C for 15min, followed by 40cycles of … WebBIOFACT can offer the best services to our customers through customized consulting services. BIOFACT has been discovering diverse products of Korean and global brands …
WebHIGene Genomic DNA Prep Kit (Biofact, Korea) and diluted to approximately 50 ng/μl for the rep-PCR templates. The genomic DNA was added for amplification using the DiversiLab Propionibacterium Fingerprinting kit (bioMérieux, France) including patented primers following the manufac- turer's instructions. According to the manufacturer's stan- WebApr 14, 2024 · To identify viruses in symptomatic Cz, total RNA was extracted from leaves of a Cz sample using the Plant RNA Prep Kit (Biocubesystem, Korea) and tested by RT …
WebFeb 28, 2024 · All qRT- PCR analyses were performed CFXrealtime PCR system CFXsystem software (Bio-Rad, CA) using Ever-Green 20 fluorescent dye (BioFACT, Daejeon, Korea). examineIbINH gene expression profiles, various tissues including leaf (5th from top),stem (4th-6th inter- node), fibrous root, pigment root storageroot were … WebBIOFACT Co.,Ltd. - Korea supplier of Taq, PCR, DNA Prep Kits, DNA Amplification, DNA Purification, DNA isolation, DNA Clean Up, Taq DNA Polymerase, Multiplex PCR, …
Web0.4mlF-starTaqDNApolymerase(BIOFACT,Korea).PCRcycling conditions were largely identical for selected genes except for the primer annealing step. All of the PCR cycles were run with an initial denaturation step at 95°C for 4 min, followed by 34 cycles of dena-turationat95°Cfor35s,primerextensionat72°Cfor35s,andafinal
WebBioFact™ 2X OneStep Multiplex qRT-PCR Kit for Probe is the best optimized to a system which is using sequence specific designed probe from various RNA samples. The … som hr self service centerWebNov 25, 2013 · BIOFACT (Previous SolGent S&C Co.,Ltd.) is a spin-off company from SolGent Co.,Ltd which has been doing a business for over a decade in Korea. Recently … small cottage interior trends 2021WebBIOFACT can offer the best services to our customers through customized consulting services. BIOFACT has been discovering diverse products of Korean and global brands in order to provide the best products to the customers. In addition, we supply above products through our unique distribution network more quickly than any other companies. small cottage kitchen designs photo galleryWebJul 1, 2024 · Total RNA was extracted from each retina using BioFACT™ Total RNA Prep Kit (Biofact, Korea) according to the manufacturer's instructions. RNA quantity and quality was evaluated with a NanoDrop 2000 Spectrophotometer (Thermo Scientific) and gel electrophoresis. 1 μg of total RNA was reverse transcribed using BioFact™ 5× RT Pre … som house shoal bayWebFeb 8, 2024 · Each 20-µl reaction mixture contained 2–4 µl template DNA (100–150 ng), 1× F-star Taq Reaction buffer (BioFact, Korea), 0.2 mM of dNTP (BioFact, Korea), 10 pmol of both primers, 1.25 U of F-star Taq DNA polymerase (BioFact, Korea) and 1 µl dimethyl sulfoxide (Sigma, St Louis, USA). Thermal cycling was conducted on an Applied … som hurricaneWebJun 10, 2024 · We performed PCR using BioFACT™ Taq DNA Polymerase (Biofact, Daejeon, Korea). The 25 µL PCR mixture was composed of 5 µL of 10× DNA polymerase buffer, 2.5 units of 10× Taq DNA polymerase, 1 µL of genomic DNA, 10 pmol of each primer, and 0.5 µL of a 0.2 M dNTP mixture. The PCR conditions were as follows: denaturing at … somi aestheticWebNov 25, 2013 · BIOFACT (Previous SolGent S&C Co.,Ltd.) is a spin-off company from SolGent Co.,Ltd which has been doing a business for over a decade in Korea. Recently … small cottage kitchen cabinets